The Chatlos Foundation provides grants to nonprofit organizations in the United States and globally, with a primary focus on supporting US-based tax-exempt organizations. Grants are typically awarded for program support, and initial requests can receive up to $10,000. The Foundation allocates its grants across several categories including strengthening Bible Colleges/Seminaries, advancing religious causes, addressing medical concerns, promoting liberal arts colleges, and tackling social issues. Funded projects should aim for self-sufficiency and reduce reliance on ongoing support.

To be eligible for grants, organizations must have been operational for at least two years and be recognized as tax-exempt by the Internal Revenue Service. The Foundation does not support organizations that engage in discrimination based on race, color, gender, creed, age, or national origin, and does not provide direct scholarship grants to individuals.

Grant proposals can be submitted at any time as there are no specific deadlines. Once submitted, a proposal undergoes a review by the Preliminary Review Committee, which meets monthly. If the proposal is deemed appropriate, it proceeds to a full board review. The entire review process usually takes around 180 days. Grantees can only receive one grant within a 12-month period and can resubmit proposals six months after a denial. Funded organizations are expected to provide reports and updates on project outcomes.

While the Foundation does not accommodate personal meetings with initial funding applicants, organizations that have received funding may schedule appointments to visit the Foundation's offices.